Intracellular pH of snail neurones measured with a new pH-sensitive glass micro-electrode

Measurement of intracellular pH of skeletal muscle with pH-sensitive glass microelectrodes.

We used three methods to examine the relationship among intracellular pH, transmembrane potential, and extracellular pH. Single-barreled electrodes permitted the determination of resting potential and intracellular pH with a minimum of cellular injury. Double-barreled electrodes, which incorporated a reference as well as a pH-sensitive electrode in a single tip, facilitated the direct measureme...

Measurements of the Intracellular Ph in a Single Cell of Nitella Flexilis by Means of Micro-glass Ph Electrodes.

The use of micro-glass electrodes sensitive to the activity of selected cations has permitted a direct measurement of intracellular ionic activity , e. g., the activity of hydrogen ion in a single crab muscle fiber (CALDWELL 1954), and in a single squid giant axon (CALDWELL 1958, SPYROPOULOS 1960), the activity of sodium and potassium ion in a single squid giant axon (HINKE 1960). With a micro-...

Determination of vaginal pH by pH indicator strip and by pH micro electrode.

Vaginal pH measurement is a screening method of vaginal infection in pregnancy. We tested two different methods of pH determination. In freshly taken vaginal fluid measurements were carried out with special pH indicator strips and pH micro glass electrode. About 100 microliters of vaginal fluid is enough when pH indicator strips are used, and 200 microliters when the micro electrode is used. Th...

pH Measurement with the Glass Electrode and Vacuum Tube Potentiometer.

pH-Sensitive Hydrogel for Micro-Fluidic Valve

The deformation behavior of a pH-sensitive hydrogel micro-fluidic valve system is investigated using inhomogeneous gel deformation theory, in which the fluid-structure interaction (FSI) of the gel solid and fluid flow in the pipe is considered. We use a finite element method with a well adopted hydrogel constitutive equation, which is coded in commercial software, ABAQUS, to simulate the hydrog...